Research Overview
Left Z-stack projection of patched cortical astrocyte. Patch pipette (green) filled with Alexa-488. Astrocytes (red) were loaded with SR101
Right Example image of astrocytes filled with fluorescent dye passing between cells.
Our lab focuses on different aspects of astrocyte-neuron interactions in the healthy brain and disease models. In particular we study the effects of stress on astrocyte structure and function. We use an array of stress paradigms in an attempt to model different aspects of stress-disorders such as anxiety, PTSD, and depression. Our goal is to reveal how the effects of stress on astrocytes influences neuronal function and output, which in turn underlies behaviour.
We are keenly interested in the link between astrocyte metabolism and synaptic plasticity, investigating the specific conditions under which neurons rely upon astrocytes for energetic substrates, gliotransmitters, as well as clearance of neurotransmitters and metabolites. The lab has active collaborations to develop the next generation of tools to understand astrocyte metabolism.
We use complimentary techniques combining two-photon microscopy, electrophysiology, in vivo imaging, and behaviour. Situated in Montréal, we have access to diverse techniques and tools in our core facilities and through our internal collaborators at the CRCHUM and Université de Montréal, as well as external collaborators at nearby universities.
Current Projects
How does stress affect astrocytes and what are the consequences for glial and neural function? This project focuses upon the central signalling cascades initiated after stress, including how stress is sensed by astrocytes, cell-specific alterations, and downstream consequences.
How do astrocytes contribute to stress-induced memory impairments? We study the role for astrocytes in memory formation, and how this can malfunction after stress. This project uses behavioural assays to determine the effects of stress on memory combined with the use of genetic tools to influence glial and neuronal function.
Regulation of astrocyte function by glucocorticoids. This project investigates how glucocorticoids alter astrocyte function and the consequences on synaptic function and behaviour.
Influence of astrocytes on metabolism and feeding. Using metabolic phenotyping, behavioural assays, and genetic manipulations we study how astrocytes in hypothalamic brain regions regulate metabolic function.
Funding sources
The lab is supported by the Canadian Insitutes of Health Research (CIHR), the Canadian Foundation for Innovation (CFI), the CRCHUM Research Centre, the CHUM foundation, the Brain and Behavior Research Foundation (NARSAD Young Investigator award), the Réseau Québécois sur le suicide, les troubles de l’humeur et les troubles associés (RQSHA), Montreal Diabetes Research Centre (MDRC), the Natural Sciences and Engineering Research Council of Canada (NSERC), and the Fonds de Recherche Québec Santé (FRQS), providing funding to investigate distinct aspects of stress, astrocyte function, and the impact on behaviour.
